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Image Search Results
Journal: Journal of Neuroscience
Article Title: In Vivo Measurement of Brain Extracellular Space Diffusion by Cortical Surface Photobleaching
doi: 10.1523/jneurosci.2294-04.2004
Figure Lengend Snippet: Figure1. InvivoloadingofbrainECSbyfluorescein-dextrans.A,Brainsurfaceexposureafter craniectomy showing cortical blood vessels and intact dura. B, Transdural loading of brain ECS showing a cylindrical dam containing aCSF solution of fluorescein-dextran. C, Fluorescence image of cortical surface after dye loading. Scale bar, 1 mm. D, Coronal 300 m brain slice obtainedexvivoafterloadingdemonstratesfluorescenceloadingofthecortex.Scalebar,1mm. A gradient of fluorescence signal is observed from the cortical surface (top left) to the dorsal hippocampus (bottom right). Arrowhead, Cortical blood vessel; asterisk, white matter.
Article Snippet: Calibration curves relating t1/2 to the relative diffusion coefficient (D/ Do) were generated by measuring t1/2 for
Techniques: Fluorescence, Slice Preparation
Journal: Journal of Neuroscience
Article Title: In Vivo Measurement of Brain Extracellular Space Diffusion by Cortical Surface Photobleaching
doi: 10.1523/jneurosci.2294-04.2004
Figure Lengend Snippet: Figure 2. Brain ECS diffusion measured by cortical surface photobleaching. A, Schematic of apparatus for cortical surface photobleaching measurements in vivo. B, Photograph showing glass window positioned at the dural surface to dampen cardio- respiratory brain oscillations. C, Representative fluorescence recovery curves for 4 kDa fluorescein-dextran in aCSF (red), brain cortex(black),andaCSFcontaining30%glycerolhavingviscosity2.7centipoise(blue;spotsize,5m).D,Left,Relationship between t1/2 (half-time) for fluorescence recovery and relative brain versus aCSF diffusion coefficient (D/Do; top x-axis) deduced fromphotobleachingmeasurementsonfluorescein-dextran-containingsolutionsmadeviscouswithglycerol(bottomx-axis).See Resultsfordetails.Right,FluorescencerecoverycurvesforaCSFcontaining4kDafluorescein-dextranandindicatedconcentrations of glycerol.
Article Snippet: Calibration curves relating t1/2 to the relative diffusion coefficient (D/ Do) were generated by measuring t1/2 for
Techniques: Diffusion-based Assay, In Vivo, Fluorescence